Saturday, May 23, 2020

Mamas Meal Product Category Restaurant And Ready to Eat Meals - Free Essay Example

Sample details Pages: 5 Words: 1443 Downloads: 4 Date added: 2017/06/26 Category Marketing Essay Type Analytical essay Did you like this example? Product name: Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s meal Product category: Restaurant And Ready to eat meals. Slogan: Yummy Food Mummy Food EXECUTIVE SUMMARY: The general image of Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s Meals will be that it products are convenient, healthy and affordable. They are easy to use, saves time and can be used with a least effort. As defined in target market section that young males who are living far away from home canà ¢Ã¢â€š ¬Ã¢â€ž ¢t cook food and eating daily from restaurants is quite expensive, so our product is convenient to consume for these users. Similarly our product will also be convenient for working class because after working half the day they donà ¢Ã¢â€š ¬Ã¢â€ž ¢t want to get engage in long cooking process, we are providing them with an alternative of home cooking. They can enjoy a delicious healthy meal which is made in no time. INTRODUCTION TO SITUATIONAL ANALYSIS The product we have chosen for our advertisement is ready made meal. This may sound confusing at first, but what ready made me is a perfectly cooked meal that has a shelf life, it is a readymade delicious meal that is already prepared for you, all you have to do is simply warm up the meal. Foreign products like Heinz has been able to do this, they have a readymade soup that is preserved in a can, all you have to do is open up the can and pour out the soup and warm it up. We have created a similar product. Just imagine if you could have Achari chicken or Briyani that is ready made, and all you have to do is warm it up, there is no involvement of preparing the food, you just have to heat it up. We want to cater those who do not have the ability and time to devote towards preparing meal , for example bachelors, student who live in hostel or those females who find it always a tedious job to cook a meal after work one just has to Simply heat up a delicious meal that they bought from their local grocery store. We chose the product because we found it to be somewhat unique then other easy to make foods, if we where to look at KnNs nuggets or Maggie noodles , these two foods involve the effort of preparing the meal, they may be simple and a 2 step process in preparing the meal but it still requires time and effort. We want our customers to feel that when they come home all they have to do is warm up a delicious meal, a meal that is so tasty and made with love it feels as if your own mother has made it for you. Our customers should feel that there is a food that will be provided to them instantly. It is a way to care for those who wish to be look after of. For bachelors it may be the nostalgic moment of having a good warm meal prepared for them and the meal tastes just as good as their mother used to prepare for them. By adding the touch of love and care of a good cooked warm meal, we have decided to name our product à ¢Ã¢â€š ¬Ã‹Å"Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s Mealà ¢Ã¢â€š ¬Ã¢â€ž ¢, the meal is so good and feels as if it has the touch of love as if your own mother has prepared it for you. We are reaching out to our customers by the emotional appeal of their want and desires by satisfying it with our product. STRENGTHS: Don’t waste time! Our writers will create an original "Mamas Meal Product Category: Restaurant And Ready to Eat Meals" essay for you Create order There is scope for of growth of ready to eat meals as the demographics and trends are changing. New demographics like two- income households and single parent households. Wide varieties of dishes are going to be offered to cater to different taste buds. Hassle free and attractive packaging of our product will set us apart from our competition. WEAKNESS: Acceptability of this type of food by the potential customers. A general perception prevails that preserved food is unhealthy. Lack of awareness about the product. OPPORTUNITIES: Poor distribution of the competitorà ¢Ã¢â€š ¬Ã¢â€ž ¢s product can act as an opportunity for us. Out of 6 local supermarkets researched only 2 had the product available. Lack of advertising by the competitor can also act as an opportunity in order to attract customers. Diversify into other business lines like frozen foods and canned foods. Export our products to countries like U.K and U.S.A which has a large market for ready to eat meals. THREATS: Only one competitor present in the market and the moment namely freshmate. Other threats include frozen foods like k nà ¢Ã¢â€š ¬Ã¢â€ž ¢s, menus, low priced food restaurants like dogar, maggi noodles. THREAT OF NEW ENTRANTS: The market of our product is such that there is some threat of new entrants because the market seems very attractive due to only one direct competitor (Freashmate) in the local market. The other attractiveness for the new entrants is that the segmentation is also much defined. The product differentiation is also another important point because there is always a chance of a new entrant introducing something new in the ready to eat meals category. Then the product is not very expensive and it is a general routine product so the switching cost of the consumers is not high. One barrier to entry is the initial set up cost which is slightly high and so it can somehow reduce the threat of new entrants. Target Market and Consumer Profile For a company to accomplish success in its marketing efforts it is important for it to realize the need for segmentation. Segmentation can be defined as dividing a market into smaller groups with distinct needs, characteristics, or behavior that might require separate marketing strategies or mixes (kotler et al.13th edition). In todays world it would be wrong to assume that a company can cater to everyone in the market hence it is important for a company new or existing to segments its markets. Segmentation can be done based on several factors namely demographics, psycho graphics, geographic and behavioral. The market can be segmented in the following categories Target Market and Consumer Profile The market can be segmented in the following categories Demographic Age(18-24), (24-36) GenderMale, female Family size3 and more persons Family life cycleSingle, Married with children Income30,000 and plus EducationCollege, University LifestyleBusy, Social Psycho graphic Social Classmiddle class, upper middle class Socio economic ClassA1, A2,B* AttitudesQuality minded, looking for convenience Behavioral Occasionsregular BenefitsGood quality, good taste, Convenience User Status First time user PRICE AND DISTRIBUTION PRICING: Price plays a very important role in the success of any product. Our product Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s meals which is a ready to eat meal has only one direct competitor in the market while there are many other close substitutes. We are going to use a competitive pricing strategy because the price of our competitor is reasonable enough and another point is that we have an edge on Freshmate due to better and more impressive packaging which will look more hygienic, so the customers can get a better product in same price as that of Freshmate. Our traditional line of Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s meals will be available in two different SKUs, a serving for 2 and a serving for 4. Same goes for the Continental and Chinese cuisine line. The price of the traditional line SKU which will serve to 2 will be between Rs.225 to 250 and the SKU which will serve to 4 will be between Rs.300 to Rs.325. The Continental and Chinese line SKU serving to 2 will range between 300 to 350 and the SKU for 4 will be within Rs.400 to 450. DISTRIBUTION: The distribution of a product matters a lot because the more convenient it is for the consumers to buy your product from the market the higher the chances of success. In the case of our product, the distribution plays a very important role because the product of our direct competitor is hardly available in the market à ¢Ã¢â€š ¬Ã…“Mamaà ¢Ã¢â€š ¬Ã¢â€ž ¢s mealsà ¢Ã¢â€š ¬Ã‚  is the product used on daily basis so we decided that we will place our product in grocery stores and bakeries as well so that the product will be in the range on our potential customers. We will also place our product in big stores or malls like CITIC Plaza, Guangzhou SM City Chengdu, Chengdu SM City Jinjiang, Jinjiang SM Lifestyle Centre, Zibo Hundred Years City We are going to use the above distribution channel because distribution in our competitive edge so we want to make sure that the product is reached to our customers and is distributed in all the places we actually want to so for that reason we are going to distribute our product our self to the retailers.

Tuesday, May 12, 2020

How Can You Help Your Child Overcome Anxiety - Free Essay Example

Sample details Pages: 6 Words: 1673 Downloads: 3 Date added: 2019/03/26 Category Psychology Essay Topics: Anxiety Essay Did you like this example? Introduction About 20% of teenagers suffer from depression on their way to adulthood and now more are due to the spread of the internet and the easy access to cyber-bullying by those they know and complete strangers as well. However while some do grow out of it without help, others need a bit of a push to get out of their mental shell keeping enclosed in something that feels like an endless hole, a constant sink. And sometimes those who need to give them that push are their parents, but how can parents really help? How can they actually put a positive impact onto this dark moment in their kids life?. Don’t waste time! Our writers will create an original "How Can You Help Your Child Overcome Anxiety?" essay for you Create order Global Perspective While this planet is evolving, so are the people living in it. In this whole world mental disabilities like depression or anxiety are sky-rocketing especially in teens between ages of 12 and older, take America, for example, according to those in the CUNY Graduate School of Public Health and the Universitys Mailman School of Public Health in Columbia, rates of teen depression and anxiety started to rise at an incredible rate within the years of 2005 and 2015. Since that was during 2015 it is most likely that, along with the advancements in technology, that by 2020 this will not only be a simple occurrence and instead become an worldwide epidemic. There are parents all over the world, and while some are living in problematic areas where they need to work more than others and dont have enough time to genuinely take care of their children, while they still do care they need to earn more money in order to support their children financially. But they can still look out for a lot of symptoms most dont realize their kids are going through, and all these slight changes are things even children themselves should be looking out for. Such as, a sudden change in the amount of food they eat, like the quantity decreasing or increasing, seeing them deny food with reasons like I lost my appetite or I already ate. This is very unhealthy but most often parents all around the world usually assume that their child is just trying out a new diet or just doesnt feel hungry in that moment. Other symptoms are like sudden outburst of anger or getting irritated or angry easier and at more insignificant things. Often having abrupt snaps at people in the ir life, distancing themselves from friends and family, keeping them at arms length. Especially with wanting their kids to go through school and have a better future than they did. But in countries that have an overall higher rate of teenagers gaining depression and anxiety parents should be looking out for the symptoms I have previously mentioned. The highest overall country with the most depression rates is India with China and USA following close behind, however for countries with higher anxiety rates the tables turn into China being first with India and USA following at 2nd and 3rd. In countries like America there are a lot of therapies that parents try to send their kids into or kids go in by their own will to help themselves with their mental problems or to get diagnosed / checkup. On one side therapy can really help a child but on the other it can simply make things worse with children feeling worse after having explained it all and putting all their fears and emotions out th ere to a stranger they hardly know. National Perspective According to the study that the World Health Organization coordinated, Indonesia, the country in which I, and many others, live in, is in the 4th place for the country with most deaths due to the behavioral disorder that is scientifically diagnosed as depression and is in 5th place for the most disease from anxiety. From an interview hosted by ncdalliance featuring the Chairman of Indonesian Psychiatric Association (PDSKJI) Eka Viora it is said that an astonishing 3.7 percent of Indonesias population has to endure depression during their youth. That is approximately 9 million people with depression and an extra 8 million whom suffer with anxiety, with some obviously being teens and children or young adults. While there are many hotlines for people who suffer from this to call, that doesnt entirely fix the problem at hand, that millions of people are under going this kind of mental unstableness that they have almost zero control since depression was studied to be relatively genetic an d so far in Indonesian and in fact the whole world , permanent cures havent been found. While anti-depressants are available they come at a price and for those who cant afford a physiatrist they cant get a prescription and therefor cant obtain the medicine. The average cost of a bottle of antidepressants like Zoloft and Prozac range from 39 dollars to a large 74 dollars. A month. While seeming like a temporary fix it also comes with several side affects such as nausea, increased appetite, fatigue and insomnia just to name a few. Personal Perspective From what I could gather from all the research I went through I found out that parents really have a very big part in someones mental health. With all of it Possible Outcomes and Scenarios There are hundreds of possible complications emerging if even a small thing was to go wrong, little ripples can create problematic waves. Many of those dilemmas is one most parents secretly fear deep down, their child committing self-sacrifice, also known as, suicide. This would be one of the worst endings to a kids life, but witnessing them struggle and go through traitorous highs and triecious lows, similar to self-harm and a constant thought of wanting to end it. With worse to come if the children are unfortunate and was gifted with parents whom couldnt care less about a childs mental state and would simply let them be, without these guardians to look up to children often fall deeper into this mayhem and end up worsening their already terrible diagnosis. A child could very well also be depressed and suffer from anxiety due to the ways their parents treated them along with the fact the most schools around the world either have horrific bullying classes which lead people to insanity and to have them jump or end it with a knife, some schools also have abusive teachers and faculty members which shouldnt be anywhere near kids. With all of these parts coming into play it almost seems like there is no escape, but truly there is, multiple ways of doing so, in fact. Possible courses of action There are many ways to nip this problem in the bud, some including anti-depressants and professional help from a therapist. Personal response I suppose my life has been pretty smooth through out it all, with a few bumps but nothing bad really happens. And Im fortunate to have parents like my own that dont cut the crap when it comes to their kid. I had a few bad days and went through my own small depression with short relapses now and then, luckily for me I never needed to take medication or needed any kind of professional help. Through out it all, I found that my friends were the biggest help and not so much my parents, while they were there for me they never really helped. I tried opening up and they didnt say much, and when I spoke about my problems they simply gave a straight forward answer and didnt help emotionally more so physically. But without them I wouldnt have become who I am now, which in the results of the 16personalities test happens to be an INTJ, introverted, nurturing, thinker and a judger. Which I can, now, proudly say I am. During the times of relapse my father tended to be on the more cold side of the s pectrum which many work on many children, apparently me as well, choosing against being a shoulder to cry on and instead being someone who would give me the cold, hard truth whether I wanted it or not. Often opting to choose telling me how to fix my problems and getting to the root of everything and telling to man up, or in my case woman up. While most of this treatment did work, I found myself falling into a deeper hole, because of envy on how others have parents who treated them sweetly. But in the end, Im glad I have my parents who pushed to my breaking point and broke me into something I never thought I could be. Similar to a Matryoshka doll. This way of reaction to when a child opens up isnt something I would recommend all parents to do, however every child is different and should be all treated with the rights of any human and as parents they should be treated with kindness and warmth as a default. Firstly parents need to have a very close relationship with their children, while parents cant expect kids to open up to them with out a very close friendship and trust, families all around the globe should already be close enough so that the members can feel safe to open up to others. Now onto how parents are supposed to deal with this, if their children show signs of possibly having it. Its natural for parents to be worried when their children display signs of unstableness or the lack of will to live, however very few times do parents actually acknowledge these little changes their child shows. While most adjustments are very common like those I have previously mentioned and are easier to notice, this reason is one that very few people realize. The blanking of the eyes, looking into the eye of someone who is going through something difficult or have lost hope often seems blank, or as if all the twinkle it once had has disappeared or vanished between a period of time. But for one to realize this disorder by themselves if often found out by the constant or reoccurring thoughts of death and suicide.

Wednesday, May 6, 2020

Building a Business Free Essays

The concept of dating and matchmaking has evolved since the dawn of the technological age. With the emergence of the Internet and the World Wide Web, people have been trying to adapt their lifestyle according to the concept of the internet. Electronic messaging and online chatting enabled business organizations to establish online dating services for people who for some important reasons are always hooked up to the internet having no time or perhaps, really unable to build up romantic relationships in the real world. We will write a custom essay sample on Building a Business or any similar topic only for you Order Now Online dating services such as eHarmony, match. com, Yahoo! Personals, American Singles, etc. reinvent the face of dating and matchmaking by allowing online users to become members of their company with the promise of finding the right match for a romantic relationship. The good thing about online dating is that people can choose who they want to get to know better just by looking at personal profiles posted with pictures, personal interests and hobbies, etc. However, meeting someone through the internet takes time and communication is limited within the range of the computer. Moreover, people can easily pretend to be someone they are not, without anyone knowing. (Dunn) Although competing against these reputable and conventional online dating services is far-fetched because of their worldwide popularity and established client base, other dating alternatives such as dating through the phone are still more convenient and personal. Why should we spend our time sitting in front of the computer waiting for online dating services to pick out people who are compatible with our profiles? The thing is even if online dating services generate a list of thousands of people who are compatible with our profiles, are we going to spend our time getting to know all these people, when time and effort should be spent on building a stable relationship that will last longer? Dating by phone is the closest alternative to personal dating. People can speak directly to each other without limits. Moreover, personal communication allows people to communicate clearly and talk about everything they are thinking of. Conversing through phone is also easier and more convenient because people are able to talk through their mobile phones while at the same time go around for other chores or activities that they want to attend. The concept of dating by phone sprang up because it seems like everyone is looking for love. If we watch television, read magazines, or just to talk to other people we know, there will always be a topic or theme about dating, matchmaking, love, relationships, and even marriage. Although there are many other phone-dating services online, such as MegaMates. com, tango personals, Live links, etc. there are still other additions and improvements that can be done in order to reinvent the existing phone-dating services available at present. The concept of dating by phone shall be established by developing a phone dating service company. It shall start as a small company that will be made available online, by telephone and mobile phone, or by personal membership, not only in a particular country but also internationally. The company shall be called â€Å"Let’s Talk About Love,† starting up with a small staff that handles personal membership, phone calls for subscriptions, and sales and advertising. People who are interested to become a member have the option to visit the company to fill up membership forms and finally for an audio recording over their profile that shall be available for match seekers who subscribe to the company as well. For callers, an 1800 number shall be made available for them. Once they call the company, they shall be asked to prompt in details such as being a first time user, answering of survey questions and audio recording their profile by phone as well. Online users who visit the website and are interested to join as members are able to fill up forms over the internet and send audio recording of their profile or to call the number of the company if they are unable to do the recording through the computer. The profiles answered through the forms and the surveys shall be kept in a single database in the computer for future reference and for matchmaking purposes. Once the company finds a match, members shall receive a call in order to listen to the audio recording of the matched person on the other line. If the person wants to get to know the other person on the line, the personal number shall be given so they can start conversing after the first call. Members can also choose to receive a list of compatible people with their profile. They can choose whose audio recorded profile to listen to and whose number to call. If the other person agrees to communicate with them, then they will start up a two-way conversation over the phone. The good thing about this concept is it leans toward the human element of personal communication. Listening to the other person speak allows people to understand and get to know what kind the other person is. In contrast, reading profiles over online dating services is impersonal and ambiguous. â€Å"Let’s Talk About Love† is different from all other phone-dating services available online for people who are looking to establish romantic relationships. The usual phone dating services are only existent online and profiles are seen only through the internet, numbers to call are made available directly to them. â€Å"Let’s Talk About Love† aims to make phone dating services more personal by allowing members to do audio recording messages of their profiles, and communication is simply done by calling. This medium becomes more personal. Moreover, the company wishes to incorporate new mobile phone technologies available nowadays. The company operates for both telephones and mobile phones. To make it more personal the company plans to team up with mobile phone providers and service network providers such as ATT, Nokia, Samsung, T-Mobile, Cingular, etc. to make â€Å"Let’s Talk About Love† services more personal and closer to face-to-face interaction. The company plans to offer personalized telephone units and mobile phones from these companies directly to members for advertising purposes and to get large companies to invest in the business. Selling phones and mobile phones from mobile phone companies allows users to purchase a phone, which is directly connected, to â€Å"Let’s Talk About Love. † For instance, just pressing the numbers 1-4-3 directly connects the caller to the voice mailbox for new messages from the company and their phone-dates. The 3G technology is also popular nowadays. Selling mobile phones with 3G technology and telephones with screens for live phone chats allows callers to see whom they are speaking to. (â€Å"3G Phones†) This is a great way to improve the usual phone dating service available for people. Aside from talking to their phone-dates, members may also get to see them. All they have to do is to purchase 3G phones manufactured by popular mobile phone companies that are tied up with â€Å"Let’s Talk About Love† in order to get full services from the company. Perhaps, partnership with mobile phone companies will allow them to produce a â€Å"Let’s Talk About Love† mobile phone edition, maybe packed with â€Å"Let’s Talk About Love† logos, wallpapers and ring tones with love themes, or mobile phones with red or pink casings, etc. This is a good form of advertising both for mobile phone companies and â€Å"Let’s Talk About Love. † Partnership with service network providers such as T-Mobile or Cingular will follow the same concept. For online advertising, teaming up with search engines such as Google or Yahoo! , like what T-mobile have done (Boswell). Once an online website is established over the Internet, teaming up with search engines allows the company to be visible for online users. Once online users search for tags such as love, dating, relationships, marriage, etc. the search engine will generate â€Å"Let’s Talk About Love† website as one of the top results. Of course, banner advertisements and pop-ups is also a good way to advertise over the internet. Works Cited â€Å"3G Phones. † 2008. Retrieved from three-g. net. 27 Apr. 2008 http://www. three- g. net/3g_technology. html. Boswell, Wendy. â€Å"T-Mobile and Google Team Up. † 30 Jul. 2005. Retrieved from About, Inc. 27 Apr. 2008 http://websearch. about. com/b/2005/06/30/t-mobile-and-google-team-up. htm. Dunn, Susan. â€Å"The Pros and Cons of Dating Online. † 2007. Retrieved from SelfGrowth. com. 27 Apr. 2008 http://www. selfgrowth. com/articles/Dunn158. html. How to cite Building a Business, Papers

Sunday, May 3, 2020

Tuberculosis

Question: Explaining the function of the target enzyme HSaD within tuberculosis (TB). Answer: Tuberculosis (TB) is a widespread bacterial disease which is caused by different strains of mycobacterium, mainly Mycobacterium tuberculosis. It is highly infectious fatal disease transmitted through various means like air, or when people having active TB infection cough, sneezes. After infecting the body, the bacterium passes through the lymph nodes and blood vessels to all parts of the body but mostly it is found in lungs. Most infections do not show symptoms, known as latent tuberculosis, here they lives in a recessive form in the body. But as the immune system get weaker, TB bacteria become dominant. In their active state they even cause death of tissue to the organs they infect. It causes death if left untreated. As the bacteria that cause tuberculosis are transmitted through air, the disease is contagious. Generally it is evident that it causes to a person who are more exposed to person having TB on a regular basis. TB can infect to any organ of the body, but mostly it occurs in the lung which is known as pulmonary tuberculosis. Extrapulmonary TB occurs outside the lungs, even though extrapulmonary TB may occur simultaneously with pulmonary TB. There are many preliminary systems of TB such as chronic cough with blood-tinged sputum, fever, night sweats, loss of appetite, chills, weight loss and fatigue, nail clubbing etc. Characteristics of the pathogen: Mycobacterium tuberculosis is a small aerobic, non-motile bacillus. This pathogen has various special characteristics for its high lipid content. It divides in extremely slow rate as compared to other bacteria which usually divide in less than an hour, whereas M. tuberculosis usually divides every 16 to 20 hours. Its outer membrane is a lipid bilayer. When a Gram stain test is performed on this pathogen, it either stains Gram positive very weakly or does not retain stain because of its high lipid and mycolic acid content of its cell wall. MTB can survive in dry conditions and withstand weak disinfectants. It can develop naturally only within the host organism but can also be artificially cultured in laboratory. This complex pathogen includes other different TB causing mycobacteria: M. bovis, M. africanum, M. canetti, and M. microti. Among these types M. africanum is not widespread, but it is found in some parts of Africa. The bacterium of tuberculosis affects our body specifically when immune system gets weaker for some reasons or our body is previously overwhelmed by other infections, it is when that the disease occurs. It mostly affects the individuals with weak immune system such as infants, old people, pregnant women, HIV positive people, people living in unsanitary conditions, cancer patients and uncontrolled diabetes patients. When living in close contact with the person already suffering from TB. People consuming excessive alcohols, drug addicted people and individuals who weigh 10% less than recommended body weight. The treatment of TB is a long process and involves strict following doctors instruction. Even if the patients feel better the full course of medicine should be completed. The WHO had given various ways to control TB that is DOTS Directly Observed Treatment, Short Course. Some newer drugs like Bedaquiline, Delaminid and PA-824 are being treated in various clinics all over India. Prevention All of us are exposed to the pathogen of TB but only individuals with less immunity in the body seem to be affected by the disease. Therefore, keeping a healthy body is the primary way to avoid it. Some of the preventions are below: All new born infant should be given the Bacillus Calmette-Guerin (BCG) vaccine to protect against TB. Keep immunity high Maintain good hygiene Early diagnosis is the preliminary way to lessen the number of deaths due to TB. Function Of Target Enzyme HSAD The main cause of death through this disease is the pathogen Mycobacterium tuberculosis which is spread all over the world. It was virtually vanished with the help of antibiotics developed in 1950s, but the disease has reappeared eventually with new forms. Increasing prevalence of multidrug resistant organisms means prioritizing identification of targets for antituberculars. For survival of the microphage 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (HsaD), part of the cholesterol metabolism operon, is essential. The C-C bond hydrolase, HsaD, has a serine protease-like catalytic triad. The role of cholesterol is important in the infection of M.tuberculosis which is becoming increasingly prominent. Cholesterol is essential for phagocytosis of M.tuberculosis by macrophage (Peyron et al., 2000) and also play a main role as an energy source for the bacterial survival in the macrophage (Van der Geize et al., 2007). Gene deletion mutants of HsaC and HsaD show that for the survival of the macrophage these enzymes are necessary (Rangarajan et al., 2005). A subfamily of / hydrolases HsaD is part of the meta-clevage product (MCP) hydrolase class of enzymes (Lack et al., 2008). HsaD catalyses the cleavage of 4,9-DHSA within the cholesterol metabolism reaction (Van der Geize et al., 2007). There are three types of inhibitors verified for activity against HsaD. The largest group is the serine protease inhibitors. Many covalent inhibitors, for e.g., phenylmethylsulphonyl fluoride (PMSF), were tested alongwith non-covalent inhibitors, e.g., benzamidine. Acetylcholinesterases are members of the / hydrolase family and catalyse its reactions via a serine protease-like catalytic triad (Shafferman et al., 1992). A range of acetylcholinesterase-specific inhibitors were also verified, e.g., neostigmine. Humans having a structural homologue of HsaD called monoglyceride lipase (MGL). It shares the whole fold as HsaD and also acts via a serine protease-like catalytic triad, like acetylcholinesterases. A number of MGL-specific inhibitors were verified, e.g., cholesterol-like pristimerin (King et al., 2009). It is said that similar inhibitors were favoured by both of the enzymes because of the hydrophobic nature of the physiological substrates of MGL and HsaD. Spectroscopic Information From (IR, H-NMR And C-NMR) Nuclear magnetic resonance (NMR) spectroscopy is the most preferred way for the metabolic analysis of bio-fluids due to its higher reproducibility, non-destructiveness, non-selectivity in metabolite detection, and the capability to directly quantify multiple classes of metabolites. Tuberculosis (TB) which is a chronic inflammatory disease followed by multisystem involvement, causes metabolic derangements in affected patients. To explore the metabolic profile of sera from TB patients a combined multivariate pattern recognition (PR) analytical techniques with 1H NMR spectroscopy was done. A total of 77 serum samples was collected from patients with TB (n=38) and healthy controls (n=39) were examined. TB patients were distinguished from controls and creating a TB-specific metabolite profile by Orthogonal partial least-squares discriminant analysis (OPLS-DA). In concentration between the two groups a total of 17 metabolites were different specifically. Serum samples of TB patients were characterized by high concentrations of 1- methyl histidine, acetoacetate, acetone, glutamate, glutamine, isoleucine, actate, lysine, nicotinate, phenylalanine, pyruvate, and tyrosine, along with low concentrations of alanine, formate, glysine, glycerolphosphocholine and low density lipoprotein relative to control subjects. This study shows that metabolic profile of sera of TB patients and shows that NMR-based methods can be separated from TB patients from healthy controls. NMR-based metabolomics has the ability to provide the best device of TB diagnosis or with therapeutic monitoring and could add the disease understanding mechanisms better. Rationale Behind Spectroscopic Assignments With 2d NMR The entire structure of triglycosyl diacyl phenol phthiocerol of the TB bacterium was clear using the 2D -IH-NMR spectroscopy, together wih its configuration of sugar residues, composition, arrangements and linkage sites of the trisaccharide and appearance of variants in the lipid moiety. Through analysis of anomeric proton resonances IH-NMR spectroscopy has been widely used as rapid, sensitive, quantitative and non-destructive to determine the sugar composition of mycobacterial mycosides. To deciphered and provide primary and secondary structural data by scalar-correlated and dipolar-correlated spectra overlapping envelopes of resonances of an oligosaccharide is allowed by 2D-NMR.